- Name: IDEA Network of Biomedical Research Excellence (INBRE)
- Project Year: 2017
- Faculty: Tony Moschetti
- Contact: Holly O'Toole
- Funded By: INBRE
Provided five undergraduate students attending Western Nevada College (WNC), the opportunity to conduct biological research focused on the discovery, characterization, and genotyping of thermophilic bacteria unique to Northern Nevada’s hot springs. Several of the undergraduate researchers were from underrepresented groups in the STEM areas. The project consisted of 4 weeks of wet lab research, an analysis of unique and potentially important sequences in silico, and construction of a poster that was presented at the annual INBRE meeting in Las Vegas. The wet lab work included on site (hot spring) bacterial sample collection and environmental measurements, sub-culturing, analysis of bacterial morphology, bacterial enzymatic assays, and DNA extraction. Upon completion of the wet lab work, the genomic DNA of a thermophilic bacterial candidate was submitted to the Nevada Genomic Center for whole genome sequencing via Illumina. Post-sequencing, required the assembly of paired-end sequences with read length of 2x-150 base pairs for an estimated 3.3 million base pair sequence. An NCBI search should identify the unique genetic identity of our native bacterial candidate, and look for possible unique genes that code for important thermostable enzymes.