- Name: IDEA Network of Biomedical Research Excellence (INBRE)
- Project Year: 2017
- Faculty: Dr. Gary Evett and Holly O'Toole
- Contact: Holly O'Toole
- Funded By: INBRE
The WNC INBRE poster presented in Las Vegas August 2017 was the first public printing of the sequence of the in-frame 222 retained bases of intron 2 (7) of the variant in humans. This research is the first to show the co-expression of these three intron-2 variants (COX-1 pi2SV, COX-1 pi2SVD9, and COX-1 pi2SVD5) in any cells. This undergraduate research was a team of six female students led by Dr. Gary Evett and Professor Holly O’Toole studying the role that these variants might play in myeloid leukemia cell differentiation. The students were exposed to and trained in a broad range of current cell biology, microbiology and molecular biological techniques used in research. Students learned to use the inverted cell microscope, dry baths, microcentrifuges, swinging bucket centrifuge, and to make broth for cells, and agar for gel plates. Students also made cell counts with the Nexelom cellometer and used TOPO-cloning to isolate DNA. Other techniques and equipment use for students included aseptic technique, micropetting, gel electrophoresis and gel loading, cell culturing, isolation of plasmids growing in bacterial colonies and reverse transcription. Students participated in original research involving a new cyclooxygenase-1 (COX-1) variant that retains part of intron-2. Almost all of the student’s time was spent at the laboratory bench working on the research project.